Expression of phosphorylated c-Fos in HRS cells is regulated by NF-κB and COX pathways. (A) Phosphorylated c-Fos expression in KM-H2 cells was reduced by treatment with 0.3 mM indomethacin. There was a concurrent increase in (B) phosphorylated-JNK and (C) phosphorylated-c-Jun expression. (D) KM-H2 cells treated with 20 μM of BAY11-7085 showed a similar reduction in phosphorylated-c-Fos expression. The TATA box served as a nuclear protein loading control. β-Actin served as a cytoplasmic protein loading control. Western blot shown is a representative of 3 independent experiments. Band density quantification was carried out using ImageJ 1.48 software (National Institutes of Health). Density of the protein band for each condition was first normalized to respective loading control, and then fold change over untreated (UT) control cells was determined. Values shown are the mean ± SEM from 3 different experiments. *Significant difference compared with UT KMH2 cells at P ≤ .05.