Generation of CD103⁺ DCs with established BMDC protocols. (A) GM-DCs and (B) FL-DCs were analyzed by flow cytometry. Histograms show CD8α-like DC marker expression (solid black line) of live-gated CD11c⁺B220^– DCs compared with isotype controls (gray overlay). (C) Clec9A vs CD103 expression of live-gated CD11c⁺B220^– FL-DCs. (D) FL-DCs and modified FL-DCs¹⁴  were compared. (E-F) BMDC cultures were established with FLT3L (FL) or a combination of FLT3L and GM-CSF (FL+GM). After 9 days, cells were harvested and replated with either FL or FL+GM. DCs were analyzed by flow cytometry on day 15. (E) Composition of DC subsets under different culture conditions. (F) Clec9A vs CD103 expression among live-gated CD11c⁺B220^– DCs. Results are representative of 3 to 4 (A-B), 3 (C-D), or 2 (E-F) independent experiments.