AZD1480 treatment inhibited both SP and PB MF CD34⁺ cells. SP or PB MF CD34⁺ cells were treated with cytokines alone or cytokines plus AZD1480 (150 nM) for 3 days and were phenotypically characterized. The absolute number of total cells (A), CD34⁺ cells (B), CD34⁺CD90⁺ (C), and CD34⁺CXCR4⁺ cells (D) generated following the culture of SP or PB CD34⁺ cells exposed to cytokines plus AZD1480 relative to that generated in the culture exposed to cytokines alone is shown.*P < .05; **P < .01; ***P < .001 (n = 10).