Defective canonical pathway activation in the presence of nonprocessable p100. (A) Analysis of B cells from II.2 relative to controls for expression of CD86, CD69, and CD83 after 24 hours of treatment with indicated stimuli. (B) Nuclear translocation of p65 (red) in HEK293 cells transfected with the indicated constructs after stimulation with TNF. A 4,6 diamidino-2-phenylindole counterstain identifies cell nuclei. (C) Summary of p65 translocation of immunofluorescence confocal.