In vivo persistence of CAR.GD2 NKT cells in a metastatic NB model. Each mouse received IV injection of 106 CHLA-255 NB cells followed by (day 21) injection of 107 CAR.GD2 NKT cells with the indicated constructs. Cell persistence was monitored by bioluminescent imaging on the indicated days. (A) Bioluminescent images of 5 mice per group are shown from 1 of 2 independent experiments. (B) Bioluminescence photon count on indicated days. Data are mean ± standard error of the mean. Data were analyzed by Student t test at each time point after a logarithmic transformation to stabilize the variance, and relevant P values are described in the text. (C) Serum was collected from mice 24 hours after NKT-cell injection and analyzed by Luminex assay. Data are mean ± SD. *P < .05; **P < .01; ***P < .001, compared with Gz group, 1-way ANOVA with Bonferoni posttest analysis.
