TEPs are enriched within the cd41:GFPmedium combined lymphoid and precursor (L+P) scatter population within the WKM. (A) Experimental schematic for the isolation and cultivation of cd41:GFPmedium cells from the combined L+P scatter fractions of adult WKM. (B) CFUs per 100 000 cd41:GFPmedium cells plated in methylcellulose only (unstimulated) or methylcellulose plus recombinant Epo, Tpo, or Epo and Tpo grown for 4 days. Red bars represent BFU-E and CFU-E gata1:dsRed+ colonies, green bars represent CFU-T cd41:GFP+ colonies, yellow bars represent small, mixed CFU-T cd41:GFP+; gata1:dsRed+ colonies, and hatched yellow bars represent large mixed CFU-TE cd41:GFP+; gata1:dsRed+ colonies. Bars represent an average of at least 3 samples, with error bars representing SD. Please see supplemental Figures 3-5 and 8 for additional data pertaining to these culture experiments.
