CLL t(2;11) translocation characterization. (A) Giemsa banding of a CLL patient karyotype; chromosomal aberrations are marked with arrows. (B) Giemsa banding of chromosomes 2 and 11 of a CLL patient is reported; a portion of the chromosome 2 q arm is translocated on the q arm of chromosome 11; the curving arrow indicates the translocation orientation. (C) Schematic representation of chromosomes 11 and 2. The PCRs of the last 2 markers used to map the breakpoint on the chromosomes are represented in this figure. The region of 650 bp between the PCR markers 2BPdown and 2BPup contains the breakpoint and maps in the intron between exon 1 and exon 2 of CXCR4. The region of 560 bp between the PCR markers 11BPup and 11BPdown contains the breakpoint that maps in the intron between exon 1 and exon 2 of MAML2. (D) Schematic representation of translocated chromosome (2;11). The breakpoint region has been amplified by a nested PCR using (2BP up nest) and (11BP up nest) primers; the resulting amplicon has been sequenced and the fusion point has been characterized (sequence is shown in reverse). Primers sequences are reported in supplemental Table 2.