Loss of the GC after RTX treatment did not reduce Tfh numbers in the lymph node or peripheral blood. (A) Flow cytometric contour plots, (B) proportion, and (C) total number of CXCR5⁺CD57⁺CD4⁺ Tfh from iliac lymph nodes taken prior to kidney transplantation in RTX-treated patients and controls. (D) Contour plots, and (E) quantitation of CXCR3⁺CCR6⁻Th1-like Tfh (top left quadrant gate, panel D), CXCR3⁻CCR6⁻Th2-like Tfh (lower left quadrant gate, panel D), and CXCR3⁻CCR6⁺Th17-like lymph node Tfh cells (lower right quadrant gate, panel D). (F) Contour plots, and (G) bar graphs of peripheral blood CXCR5⁺CD4⁺ Tfh-like cells from the same patients in (A-E). In graphs, one symbol represents one individual, and the height of the bar represents the mean.