Boc2 blocks the protective effects of ASA in a 2-event model of TRALI. BALB/c WT mice were treated with LPS (0.1 mg/kg, intraperitoneally) and 24 hours later were challenged with H2Kd mAb (1.0 mg/kg, intravenously) and euthanized 2 hours later. (A) Plasma 15-epi-LXA4 levels in mice with TRALI + vehicle, ASA, or ASA + Boc2. ****P < .0001 vs vehicle group. (B) Extravascular lung water, (C) EVPE, and (D) hematocrit in mice with TRALI + vehicle, ASA, or ASA + Boc2. *P < .05, **P < .01, and ****P < .0001 vs vehicle groups. †P < .05 and ††††P < .0001 vs ASA + Boc2 groups. (E) NPAs in the lung digestion in mice with TRALI + vehicle, ASA, or ASA + Boc2. *P < .05 and ***P < .001 vs vehicle group. †P < .05 vs ASA + Boc2 group. (F) Survival curves in mice with TRALI + vehicle, ASA, or ASA + Boc2. **P < .01 vs vehicle group and †P < .05 vs ASA + Boc2 group. Data are mean ± SD of 5 to 10 animals per group.
