Treatment with 15-epi-LXA4 decreases LPS-induced ALI and regulates NPA formation in vivo and in vitro. (A-B) ASA or 15-epi-LXA4 treatment (at doses indicated) 2 hours before and 24 hours after LPS challenge and (A) leukocyte recruitment into the air spaces and (B) BAL total protein. ***P < .001, ****P < .0001 vs vehicle groups. (C) Whole blood NPAs in mice challenged with LPS ± ASA or 15-epi-LXA4 (5 µg). **P < .01 and ****P < .0001 vs vehicle. (D) Neutrophils and platelets isolated from WT or Fpr2/3−/− mice were incubated with LPS (5 µg/mL) for 1 hour to produce NPAs in vitro. LPS induced NPA in all combinations of WT and Fpr2/3−/− neutrophils/platelets. *P < .05 vs basal (no LPS). Pretreatment with 15-epi-LXA4 reduced NPA formation after LPS in only the WT neutrophil/WT platelet group. †P < .05. Data are (A-C) mean ± SD of 5 animals per group or (D) mean ± SD of ≥3 independent experiments.
