Blocking the lipoxin receptor reverses ASA protective effects. (A) 15-Epi-LXA4 levels in plasma and (B) cell-free BAL of WT mice challenged with PBS or LPS + ASA vs vehicle (DMSO). ****P < .001 vs PBS and vehicle groups. (C) BAL WBC and neutrophil counts, (D) BAL cell-free total protein, and (E) whole blood NPAs in WT mice challenged with LPS + ASA vs vehicle or ASA + Boc2. ***P < .001 and ****P < .0001 vs vehicle groups, respectively. †P < .05 and ††P < .01 vs ASA + Boc2 groups, respectively. (F) 15-Epi-LXA4 levels in plasma of Fpr2/3−/− mice challenged with PBS or LPS + ASA vs vehicle (DMSO). ****P < .0001 vs PBS and vehicle groups. (G) BAL WBC and neutrophil counts, (H) BAL cell-free total protein, (I) NPAs in whole blood, and (J) NPAs in BAL cells of Fpr2/3−/− mice challenged with PBS or LPS + ASA vs vehicle. *P < .05, **P < .01 vs PBS. NS, not significant. Data are mean ± SD of 5 to 7 animals per group.
