Overexpression of Pyk2 promotes MM tumor growth in vivo. (A) The Pyk2 mRNA level is increased in GFP⁺/Luc⁺-MM.1S Pyk2-overexpressing cells. (B-C) SCID/Beige mice were injected IV with GFP⁺/Luc⁺-MM.1S Pyk2-overexpressing cells (3 × 10⁶/ mouse) to establish the MM xenograft model (n = 7 per group). Tumor burden was detected and quantified using BLI. Overexpression of Pyk2 significantly promoted MM tumor growth compared with vector control. Quantification of tumor burden has been detected by BLI (bars indicate mean ± SD; P indicates P values). (D) Mice bearing Pyk2-overexpressing cells exhibited poor survival compared with vector control mice (P = .006).