Simultaneous overexpression or inhibition of JARID2 and miR-155-5p in normal CD34⁺ cells. (A) Rescue of JARID2 expression in miR-155-5p–overexpressing cells. (i) Flowchart listing the experiment timing (expressed in days) after NGFR⁺ cell purification. (ii) Expression levels of JARID2 in CB CD34⁺ cells after NGFR⁺ cells purification. The JARID2 expression was measured by qRT-PCR, and data are reported as RQ. (iii) Percentage of viable CD41⁺ cells in the MK unilineage culture assessed by flow cytometry at days 4, 7, and 11 after NGFR⁺ cell purification. (iv) Results of the statistical analysis of the collagen-based clonogenic assay. The cells were plated 24 hours after NGFR⁺ cell purification and scored after 12 days. Values are reported as mean ± SEM. **P < .01; *P < .05. The results come from 3 independent experiments. (B) Simultaneous downregulation of JARID2 and miR155-5p in CB CD34⁺ cells. (i) Flowchart reporting the experiment timing (expressed in days) after the last nucleofection. (ii) Expression levels of JARID2 in CB CD34⁺ cells at 48 hours after the last nucleofection. The JARID2 expression was measured by qRT-PCR and data are reported as RQ. (iii) Percentage of viable CD41⁺ cells in the MK unilineage culture assessed by flow cytometry at days 8, 10, and 12 after the last nucleofection. (iv) Results of the statistical analysis of the collagen-based clonogenic assay. The cells were plated 24 hours after the last nucleofection and scored after 12 days. Values are reported as mean ± SEM. **P < .01; *P < .05. The results come from 3 independent experiments.