Validation of the selected genes and miRNAs in granulocytes and plasma from PMF patients. (A) Expression of the 7 selected genes in granulocytes from PMF patients and healthy donors. Gene expression levels were measured by qRT-PCR starting from granulocyte total RNA and were expressed as relative quantity (RQ). Boxes represent the interquartile range that contains 50% of the subjects, the horizontal line in the box marks the median, and the bars show the range of values. Data are representative of 32 PMF and 12 control (CTR) samples. (B) Serum levels of 2 secreted proteins (LCN2 and OLFM4) in PMF patients and healthy donors. Protein levels were measured by enzyme-linked immunosorbent assay (ELISA) and were expressed as ng/mL. Boxes represent the interquartile range that contains 50% of the subjects, the horizontal line in the box marks the median, and the bars show the range of values. Data are representative of 30 PMF and 8 CTR samples. (C) Expression levels of the 8 selected miRNAs in granulocytes from PMF patients and healthy donors. The miRNA expression levels were measured by qRT-PCR starting from granulocyte total RNA and were expressed as RQ. Boxes represent the interquartile range that contains 50% of the subjects, the horizontal line in the box marks the median, and the bars show the range of values. Data are representative of 32 PMF and 12 CTR samples. *P < .05 vs CTR.