The inhibition of P2X1 affects fibrin generation and thrombus formation in WT mice. (A) Fibrin generation and thrombus formation at the site of the laser-injury on the cremaster in WT mice in presence of absence of NF449. The median of fibrin generation and platelet accumulation during thrombus formation was calculated based on 34 thrombi for each condition performed in 3 mice. (B) PMNs were detected using the Ly-6G–specific antibody before or after infusion of NF449. The median of the integrated fluorescent signal was calculated for the 2 conditions (29 thrombi in 3 mice for each condition).