Cytotoxin injury of glomerular endothelium induces glomerular microvascular injury. (A-C) Mice were treated as in Figure 1, then frozen sections of kidney were stained with anti-mouse fibrinogen/fibrin antibody. The fluorescence intensity among regions of interest drawn around Bowman’s capsule containing the glomerular microvessels (white line) was measured using ImageJ (D). Each bar represents mean ± SEM; n ≥ 5 mice per group; *P < .05, toxin-treated groups vs PBS control group. (E) Quantification of EC transcripts from total RNA extracted from the renal cortex. The real-time PCR data were analyzed using the δCt method. Each bar represents mean ± SEM; n ≥ 5 animals per group; *P < .05, toxin-treated groups vs PBS control group.