Platelet accumulation is reduced in P2X1^−/− mice following a laser-induced injury on the cremaster. (A) Representative composite images of fluorescence and brightfield data depicting thrombus formation (in red) in WT (upper panel) and in P2X1^−/− mice (lower panel). (B) The median of platelet-integrated fluorescence (y-axis) based on 29 thrombi was performed in 3 WT mice and 30 thrombi in 3 P2X1^−/− mice and were represented over time. (C) The graph depicts the distribution of the time to acquire the maximal size for each thrombus in WT and P2X1^−/− mice. NS (P < .05). (D) The graph depicts the maximal of integrated fluorescence intensity for each thrombus in WT and P2X1^−/− mice. *P < .05. (E) The quartile distribution of the maximal integrated platelet fluorescence for each thrombus in WT and P2X1^−/− mice. A total of 29 thrombi in WT mice and 30 thrombi in P2X1^−/− mice were ranked, and the percentage of thrombi of each genotype was determined independently in each quartile of the rank order. Black bar, WT mice; gray bars, P2X1^−/− mice.