Plasma lexaptepid and iron parameters during experimental human endotoxemia. (A) Drug concentration in lexaptepid-treated subjects only. (B) Concentration of hepcidin in lexaptepid- and placebo-treated subjects. (C) Serum iron concentrations. Black arrow indicates the primary end point: change in serum iron levels at 9 hours after LPS administration. (D) Transferrin saturation (TSAT). Data are expressed as mean ± SD. Changes in all parameters over time were analyzed with 2-way analysis of variance with repeated measures, interaction term. Interaction terms are displayed within each graph. In the lexaptepid-treated subjects, hepcidin levels were still elevated at T = 168 hours (day 8) compared with baseline values (Student paired t test: P = .005).