SOX11 induces the expression of proangiogenic factors and promotes angiogenesis in xenograft MCL-derived tumors. (A) GSEA analysis on expression data set from SOX11-positive and SOX11-knockdown xenograft tumors showing enriched gene sets related to angiogenesis pathways. NES, P-val, and FDR are represented. Statistical significance is considered when FDR <0.2. (B) Top panel, Representative images showing human-specific angiogenesis antibody array membranes incubated with protein extracts from SOX11-positive (n = 2) and SOX11-knockdown (n = 2) xenograft tissue lysates. Bottom panel, Quantification of the mean pixel densities showing 21 angiogenic proteins significantly upregulated in SOX11-positive compared with SOX11-knockdown xenografts. (C) Top panel, Macroscopic appearance and consecutive histological sections from representative SOX11-positive and SOX11-knockdown xenografts (Z138 shControl, n = 3; shSOX11.1, n = 5; and shSOX11.3, n = 5) stained with a specific antibody anti-human SOX11 (×40) and a specific antibody anti-mouse CD31 (×20). Bottom panel, Density (% of CD31-positive microvessel areas) of CD31-positive MVD areas in SOX11-positive and SOX11-knockdown tumors delineated by the presence of CD31-positive staining. Bar plot represents the mean percentage ± SD. P-val are shown. The significance of difference was determined by the independent samples Student t test. FDR, false discovery rate; NES, normalized enrichment score; P-val, P value.