B-cell population decreases after bortezomib treatment. Irradiated BALB/c mice transplanted with bone marrow and spleen cells were injected with either bortezomib (0.1 mg/kg) or vehicle control starting at day 20. (A) Spleen cells were collected at day 55 and stained for CD45⁺CD19⁺ populations. (B-C) Data showing B-cell populations as percentage and total numbers from spleen. (D) Total numbers of CD45⁺CD19⁺MHC II⁺ B-cell populations in the skin samples harvested on day 55. (E) reverse transcriptase-polymerase chain reaction results detecting BAFF gene expression levels from skin samples collected at day 55. (F) Serum BAFF levels were detected by enzyme-linked immunosorbent assay and calculated as log (BAFF/B cell) ratio. (G) Total numbers of CD45⁺B220⁺PNA⁺ germinal center B cells in the spleen. All the data were collected from 2 independent experiments with 8 mice per group. The data are shown as mean ± SEM and analyzed by Student t test to compare between individual groups. *P < .05 and **P < .01 were considered significant.