The role of 12-LOX in regulating the FcγRIIa signaling complex. Washed human platelets were treated with ML355 (20 μM) or vehicle control prior to stimulation by crosslinking (IV.3 + GAM). Immunoprecipitation of FcγRIIa was conducted at 15, 30, and 60 seconds post-crosslinking, and phosphorylation of FcγRIIa was measured via western blot. The bar graph shows immunoprecipitated FcγRIIa that had been treated with DMSO or ML355 following FcγRIIa crosslinking and was assessed for phosphorylation (n = 7). Error bars indicate SEM.