Suppression of inhibitor formation against FVIII in BALB/c mice with hemophilia A by oral administration of a 1:1 mixture of bioencapsulated CTB-C2 and CTB-HC FVIII antigens. (A) Feeding and FVIII treatment schedule. Number in circle indicates time point for tail bleed. (B) Inhibitor titers (in BU per milliliter) after 4 weekly IV injections of BDD-FVIII in No plant, WT plant, and FVIII plant fed groups. IgG1 (C), IgG2a (D), and IgG2b (E) titers against FVIII for the same experimental groups. All data are shown for individual mice and as averages ± SEM. Unpaired 2-tailed Student t tests were used to calculate P values (*P < .05, ** P < .01).