Ncor2 represses fos expression in cooperation with Hdac3. (A) The expression pattern of fos in the control or ncor2 morphants at 26 and 36 hpf. (B) qPCR assay revealing the expression level of fos in the control or ncor2 morphants at 26 and 36 hpf (mean ± standard deviation [SD], n = 3, *P < .05). (C) The expression pattern of fos in hdac3 morphants and runx1 in hdac3 morphants or TSA-treated embryos. (D) FISH result indicated that ncor2 is coexpressed with hdac3 in the blood vessel region. (E) ChIP assay. The diagram depicts the location of primers used in the ChIP assay (upper panel). The pair of acetylation detection primers (fos-ac-1F/R) spans the transcription start site whereas the control pair (fos-ac-CF/R) is located about 700 bp upstream of the transcription start site. The ChIP result showed the acetylation state of the hyper-acetylation region (fos-ac-1F/R) and the upstream control region (fos-ac-CF/R) of the fos promoter (mean ± SD, n = 3, *P < .05) in ncor2 morphants, hdac3 morphants, and embryos coinjected with a low dose of ncor2 and hdac3 MO (lower panel), respectively.