RUNX1 controls transcription of Hmga2 via its promoter in a tissue-specific manner. (A) Diagram of promoter-luciferase construct showing RUNX consensus Sites 1 and 2(*) at bp −363 and −213, respectively. (B) NIH3T3 and 293T cell lines were transfected with the full-length Hmga2 promoter-luciferase construct, and luciferase assays were performed 24 hours after transfection. Coexpression was conducted with an empty expression vector (Control) or RUNX1 and CBFβ expression vectors. Error bars indicate standard deviation and are from at least 3 replicates. (C) K562, Jurkat, and U937 cell lines were transfected with the full-length Hmga2 promoter-luciferase construct, and luciferase assay was performed 24 hours after transfection. Coexpression was conducted with an empty expression vector (Control) or RUNX1 and CBFβ expression vectors. Error bars indicate standard deviation and are from at least 3 replicates. (D) NIH3T3 cells were transfected with promoter-luciferase constructs with Site1, Site2, or both sites mutated. Luciferase assays were performed 24 hours after transfection. Coexpression was conducted with an empty expression vector (Control) or RUNX1 and CBFβ expression vectors. Error bars indicate standard deviation and are from at least 3 replicates. (E) U937 cells were transfected with the constructs indicated in (D) and luciferase assays were performed 24 hours after transfection. Coexpression was conducted with an empty expression vector (Control) or RUNX1 and CBFβ expression vectors. Error bars indicate standard deviation from at least 3 replicates. *P < .05.