Figure 4
Detection of spontaneous phosphorylation of KIT, AKT, and STAT5 in ROSAKIT D816V cells by western blotting. Cell lysates from unstimulated ROSA cell lines and from the HMC-1.2 cell line or from the same cells stimulated for 10 minutes with rhSCF were subjected to electrophoresis in sodium dodecyl sulfate polyacrylamide gel electrophoresis and treated with Abs against (A) anti-human total KIT or p-KIT Y719, (B) anti-human total AKT or p-AKT S473, or (C) anti-human total STAT5 or p-STAT5 Y694. (D) An anti-human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. The lanes were run on the same gel but were noncontiguous.
