Effects of CDK6 suppression in AML cells. (A) Flow cytometric analysis of cell-cycle progression. Numbers indicate percentages of cells in G0/G1. (B) Flow cytometric analysis of apoptosis. Numbers indicate percentages of cells. (C) Flow cytometric analysis of myeloid differentiation in AML cell lines. Numbers indicate percentages of cells. (D) Microscopic analysis of May-Grünwald-Giemsa–stained cytospin preparations of AML cell lines. Original magnification, ×400. Insets show twofold magnified details of the corresponding photographs. (E) Inhibition of myeloid differentiation by expression of the CDK6 coding sequence in NOMO-1 cells transduced with an shRNA targeting the CDK6 3′ UTR. (F) Immunoblot analysis of cells shown in panel E.