Metabolic alteration of AML. OPLS-DA score plots showed a global metabolic difference between AML and HC in the training set (A) and validation set (B). (C) Metabolomic profiles of 9 AML subtypes based on WHO classification. (D) Metabolomic profiles of different cytogenetic risk groups of AML patients. (E) Heat map showed 6 differentially expressed serum metabolites involved in glucose metabolism in AML compared with HC. gr1, AML with t(15;17); gr2, AML with t(8;21); gr3, AML with t(16;16); gr4, AML with minimal differentiation; gr5, AML without maturation; gr6, AML with maturation; gr7, acute myelomonocytic leukemia; gr8, acute monoblastic/monocytic leukemia; g9, acute erythroid leukemia.