Erythropoiesis is defective in elavl1a morphants, regardless of p53 status. Whole-mount in situ hybridization was performed detecting hbbe3 transcripts at 24 hpf (A-B) or 48 hpf (C-D). Shown are representative embryos injected with elavl1a MO that displayed reduced hbbe3 transcript levels (27 of 33), compared with normal controls (38 of 40). (E-F) o-Dianisidine staining was used to detect hemoglobinized cells at 48 hpf. The representative elavl1a morphants show strikingly reduced hemoglobin staining (40 of 46), compared with normal embryos injected with control MO (60 of 60). (G-L) The same analyses were carried out using p53^−/− embryos. All embryos are oriented lateral with anterior to the left.