Figure 1
Figure 1. Development and characterization of a cyclin D1 transgenic mouse line bearing Bim−/− B cells. (A) Schematic of the cyclin D1 transgene, floxed Bim locus, and CD19CRE locus. (B) Reverse transcriptase-PCR of spleen RNA for the transgenic cyclin D1 locus in wild-type (WT) and EμCycD1 mice. (C) Western blot of cyclin D1 in spleens and thymi of WT and EμCycD1 mice. (D) Western blot of cyclin D1 in B220-sorted splenocytes stimulated with lipopolysaccharide. (E) CD19CREBimfl/fl and EμCycD1CD19CREBimfl/fl mice manifest similarly elevated WBC counts at 20 to 25 weeks of age compared with EμCycD1 mice. (F) CD19CREBimfl/fl and EμCycD1CD19CREBimfl/fl mice exhibit a similar degree of splenomegaly compared with EμCycD1 mice. *P < .05; **P < .01; ***P < .001; 2-tailed Student t test. mRNA, messenger RNA.

Development and characterization of a cyclin D1 transgenic mouse line bearing Bim−/− B cells. (A) Schematic of the cyclin D1 transgene, floxed Bim locus, and CD19CRE locus. (B) Reverse transcriptase-PCR of spleen RNA for the transgenic cyclin D1 locus in wild-type (WT) and EμCycD1 mice. (C) Western blot of cyclin D1 in spleens and thymi of WT and EμCycD1 mice. (D) Western blot of cyclin D1 in B220-sorted splenocytes stimulated with lipopolysaccharide. (E) CD19CREBimfl/fl and EμCycD1CD19CREBimfl/fl mice manifest similarly elevated WBC counts at 20 to 25 weeks of age compared with EμCycD1 mice. (F) CD19CREBimfl/fl and EμCycD1CD19CREBimfl/fl mice exhibit a similar degree of splenomegaly compared with EμCycD1 mice. *P < .05; **P < .01; ***P < .001; 2-tailed Student t test. mRNA, messenger RNA.

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