Effect of methylation (<20% vs ≥20%) on time from diagnosis to first treatment in subgroups of patients segregated by IGHV mutational status, CD38 expression, and ZAP-70 protein expression. (A) Methylation levels segregate patients who are M-IGHV (P = .02) or U-IGHV (P = .02). (B) Methylation levels segregate patients who are CD38 negative (P < .0001) or positive (P = .03). (C) Methylation levels segregate patients who are ZAP-70 negative (P < .0001) but not patients who are ZAP-70 positive (P = .27). M-IGHV, IGHV mutated; U-IGHV, IGHV unmutated.