Hemolysis rate, red blood cells loss, and biochemical changes with washing of canine blood during the 6-week storage period. Serial changes in stored blood components during 6 weeks. Serial values of (I) percent hemolysis rate (calculated by dividing the supernatant hemoglobin levels by the total sample hemoglobin levels and multiplying by 100 minus the hematocrit value); (II) the change in hemolysis rate with washing; (III) red blood cells loss with washing and (IVA-J) biochemical changes with washing of canine blood of 1 through 6 weeks of storage period are shown. Two bags of canine universal donor leukoreduced blood were sampled each week before and after washing. In panels I-III, each dark circle represents an individual bag of ∼250 g of packed red blood cells. In panel IV (A-J) individual mean values (2 bags) of the different biochemical parameters before washing are represented as dark circles and after washing as white circles.