Mass spectrometric analysis of Fc glycopeptides of total IgG1 and anti-HPA-1a IgG1 from 2 pregnant women. One person showed high degrees of fucosylation for total IgG1 (97%; A) and anti-HPA-1a IgG1 (86%; B). The other also showed a high degree of fucosylation for total IgG1 (86%; C), yet a low degree of fucosylation for anti-HPA-1a IgG1 (9%; D). Major fucosylated glycoforms are labeled in red, and nonfucosylated glycoforms are labeled in blue or are unlabeled. Blue square indicates N-acetylglucosamine; red triangle, fucose; green circle, mannose; yellow circle, galactose; purple diamond, N-acetylneuraminic acid; pep, peptide moiety; and asterisk, contaminant. Triple-protonated glycopeptide signals were observed throughout. For the assignment of the glycopeptides signals, see Table 1. The level of galactosylation, sialylation, bisection (bisecting N-acetylglucosamine), and fucosylation were calculated according to the following formulas: Galactosylation = (G1F + G1FN + G1FS + G1FNS + G1) × 0.5 + G2F + G2FN + G2FS + G2FNS + G2 + G2S. Agalactosylated structures = G0F + G0FN + G0. Digalactosylated structures = G2F + G2FN + G2FS + G2FNS + G2 + G2S. Sialylation = G1FS + G2FS + G1FNS + G2FNS + G2S. Bisection = G0FN + G1FN + G2FN + G1FNS + G2FNS. Fucosylation = G0F + G1F + G2F + G0FN + G1FN + G2FN + G1FS + G2FS.