The B abortus–injected mice develop anemia and iron restriction. Compared with saline-injected mice, the B abortus–injected mice develop (A) a significantly lower hemoglobin by 7 days, with a nadir at 14 days and partial recovery by 28 days; (B) decreased serum iron concentrations through most time points, beginning at 3 hours, except for the transient underutilization of iron around day 3 during erythropoiesis suppression; (C) decreased MCVs within the first week; and (D) elevated ZPP levels between 14 and 28 days during the time of recovery from anemia. Saline and B abortus groups each included 4 to 8 evaluable male mice per time point. Means ± standard deviation (SD) are shown; *P < .05 and **P < .001 by Student t test or Mann-Whitney U test.