BA-treated mice show signs of iron-restricted erythropoiesis. (A) mRNA level of Hamp in liver. Mouse glyceraldehyde-3-phosphate dehydrogenase and β-actin were used as endogenous controls in the quantitative polymerase chain reaction (n = 4-8). (B) Iron concentration and (C) Tf saturation in serum samples from WT, IL-6-KO, and Hamp-KO mice (n = 5-10). P values were calculated using unpaired, 2-tailed Student t test. *P < .05; **P < .01; ***P < .001.