Asxl1^−/−HSC/HPCs had an altered expression of genes implicated in apoptosis regulation and reduced global levels of H3K27me3 and H3K4me3. (A) The mRNA expression of HoxA5/7/9/10, Bcl2l13, Bcl2, and Bcl2l12 in the BM Lin^–c-Kit⁺ cells of WT (n = 4) and Asxl1^−/− (n = 4) mice were determined by qPCR. Data are shown as relative expression units to the respective gene expression in WT mice using Gapdh as an internal calibrator. (B) Western blot analyses of H3K27me3 and H3K4me3 in the BM Lin^–c-Kit⁺ cells of each genotype of mice. Total H3 levels served as loading controls. Representative blots from 3 independent experiments are shown.