Procasp8 presents as a dimer in B-CLL cells. Cytosol of 15 × 106 freshly isolated B cells from 2 normal donors and 14 B-CLL patients equally distributed between the UM-CLL and M-CLL subsets underwent a linear glycerol gradient (10% to 40%) and was centrifuged 18 hours at 100 000g in an SW60Ti rotor (Beckman Coulter) at 4°C. (A) Eighteen fractions (200 μL each) of the glycerol gradient were collected from top and underwent Wb analysis with anti-p-procasp8, anti-procasp8, and anti-Lyn antibodies. Densitometric analysis (arbitrary units) of the Wb bands of the immunoblots from 2 normal donors and 14 B-CLL patients equally distributed between the UM-CLL and M-CLL subsets are shown. (B) Wb analysis of one normal donor compared with that of UM-CLL (no. 5) and M-CLL (no. 13) subsets are shown.
