Influence of ADAM10 or ADAM17 deficiency on cell recruitment to lung tissue and on edema formation. (A-F) Vav-Adam10^−/− mice, Vav-Adam17^−/− mice, and respective litter controls were intranasally treated with 400 µg/kg LPS or vehicle (PBS). Three-micrometer sections of formalin-fixed and paraffin-embedded lung tissue were stained with hematoxylin-eosin to differentiate monocytic and polymorphnuclear cells. Representative overview images are shown for (A) Vav-Adam10^−/− mice and (B) Vav-Adam17^−/− mice (upper). Alveolar lumen (Al), neutrophils (filled arrowheads), and monocytic cells (open arrowheads) were marked in the high magnifications (lower). Ten images per animal were analyzed for the recruitment of (C) neutrophils and (D) monocytes using AixoVision software. Twenty-four hours after application, (E) alveolar protein influx was determined and (F) lung wet-dry-ratio was determined. Quantitative data represent means ± SEM (n = 5). Significance was calculated using 1-way ANOVA and the Bonferroni post-test and is indicated by crosses. Crosses without a line indicate significance to the PBS-treated control. Bar legends are the same for C and E and D and F.