PVM infection in the Af allergen-driven mouse airway inflammation model. (A) The Af sensitization, challenge, and PVM infection protocol used for these experiments. i.p., intraperitoneal inoculation; i.n., intranasal inoculation. The protocol for ova sensitization and challenge included i.p. inoculations on days 32 and 18, with all else remaining as shown. (B) Total eosinophils in BAL fluid from Af-sensitized and -challenged, virus-naïve, and PVM-infected B6 mice at day 4 after virus inoculation. (C) Cell differentials from mice in (B), as well as controls that were Af sensitized but not Af challenged. (D-E) Microscopic histology of lung tissue from mice that were Af sensitized and challenged and PVM infected (day 6 after PVM inoculation; see [A] for protocol); original magnifications ×10 and ×40, respectively. (F) Microscopic histology of lung tissue from mice that were Af sensitized but not Af challenged prior to PVM infection (day 6 after PVM inoculation); original magnification ×10.