![The liver-specific KO mice recapitulate the phenotype of the total KO mice. (A) FPN and ferritin expression in the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates by western blotting. (B) Immunohistochemistry for FPN in the duodenum of Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 50 μm. (40/0.60, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (C) Western blotting in duodenum scrapings of 6-month-old Hepc∆total, Hepc∆liver, and control mice. (D) Liver, pancreas, and plasma iron in 2-, 3-, and 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates. n > 5. *P < .05, ***P < .001. ns indicates not significant. Perls’s blue staining of the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 100 μm. (20/0.4, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (E) Plasma hepcidin in 6-month-old control, Hepc∆total, and Hepc∆liver mice. n = 4.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/123/23/10.1182_blood-2014-01-550467/4/3646f2.jpeg?Expires=1722197556&Signature=LbrStcwytkVWdzj12ceww~oIxabaEvgvCq1hpnnNvCUlmekyEFHfPV2IhVkPMrjjTxRHHvTVBIELeJWERXKxtkUPTt2PkUdN4bdMlnnjKlA8DkZGhrhU81GtiHL9CiwvTPXYaF1syDN7Al4tPK0CSwOINlt~Kxh5KbIuExLoW60eWcI4NjYO7GecZ9TStaSeYMY3iSa18zy6NRS8s~msGZcDYSsRjxviyZVPuJA1nvNnJ-DANjm1PGp9TbOs61LDwBMFfAW1yXzVGqGFctrCSqDe24R-LFlfPisAYa35Qbmh8lkNhTBgqu-qVzio3u6Pvf6PqbXHz6NQQgPZ~LJ1Fw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The liver-specific KO mice recapitulate the phenotype of the total KO mice. (A) FPN and ferritin expression in the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates by western blotting. (B) Immunohistochemistry for FPN in the duodenum of Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 50 μm. (40/0.60, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (C) Western blotting in duodenum scrapings of 6-month-old Hepc∆total, Hepc∆liver, and control mice. (D) Liver, pancreas, and plasma iron in 2-, 3-, and 6-month-old Hepc∆total and Hepc∆liver mice and their control littermates. n > 5. *P < .05, ***P < .001. ns indicates not significant. Perls’s blue staining of the spleen of 6-month-old Hepc∆total and Hepc∆liver mice and WT mice. Bars represent 100 μm. (20/0.4, Leica DMI3000B microscope, Leica DFC310FX camera [Leica LAS Core software]). (E) Plasma hepcidin in 6-month-old control, Hepc∆total, and Hepc∆liver mice. n = 4.
