BMS936564-MDX1338 targets C1013G/CXCR4-mutated cells in vivo and targets survival- and apoptosis-related signals in WM cells. (A-E) BMS936564-MDX1338 inhibited dissemination of C1013G/CXCR4-mutated WM cells in vivo. Human CD20 and CXCR4 staining was significantly reduced in tissues explanted from mice treated with BMS936564/MDX1338 compared with isotype control-treated mice (untreated). P indicates P values. Representative immunohistochemistry images are shown in supplemental Figure 6. (F) C1013G/CXCR4-mutated cells have been cultured in presence or absence of BMS936564/MDX-1338 for 6 hours, showing inhibition of phospho(p)-ERK, ERK, p-AKT, AKT, and p-Src. Similarly, cells were exposed to the compound for 14 hours, and induction of apoptosis-related pathways was documented (p-GSK3β, p-β catenin, PARP, and Caspase-9).