c-Myc is a direct target of ULBP1 and ULBP3. (A) Schematic picture of the potential c-Myc BS in the ULBP1/3 genes. (B-C) Binding of c-Myc to the potential c-Myc BS in the ULBP1/3 gene promoters was analyzed in KG-1(R), and primary blasts were or were not treated with c-Myc inhibitor (10058-F4) by ChIP assay. The amount of immunoprecipitated chromatin bound by either isotype control or c-Myc mAb was quantified by real-time PCR with specific primers. Specific signals were set relative to signals obtained for the input chromatin. One representative experiment of 2 ChIP independently conducted experiments is shown. Data were analyzed with GraphPad Prism. Two-tailed Student t test was used for single comparisons. Statistically significant differences are indicated by asterisks (*P < .05; **P < .005; ***P < .0005).