Expression of pro- and antiapoptotic genes, c-Myc, AKT, and ERK in cytarabine-resistant cells. (A) Pro- and antiapoptotic gene transcript quantification by qPCR using a 94-gene apoptosis dedicated array. Results are representative of 2 independent experiments. (B) Western blot analysis for c-Myc and constitutive AKT and ERK activation was assessed using antibodies that recognize AKT phosphorylated at ser-473, and ERK1/2 phosphorylated at Thr202/Tyr204 on whole cell extracts. Actin was used as a protein loading (40 mg per lane; representative experiment of n = 3).