Effect of autologous T cells on AMG 330-induced cytotoxicity in primary AML cells. (A-B) To test the effect of autologous T cells on AMG 330-induced cytotoxicity of primary human AML cells, frozen aliquots of pretreatment specimens from 2 AML patients were used; both patients presented with hyperleukocytosis, and research specimens were obtained via leukapheresis. One portion of the aliquot was labeled with CellVue Burgundy, and lymphocytes were isolated by fluorescence-activated cell sorting based on CD45 and side scatter properties. A second portion of the aliquot was left untreated and subsequently incubated with increasing concentrations of AMG 330 in the absence or presence of additional isolated autologous T cells in defined E:T cell ratios. After 48 hours, cell counts were determined and cytotoxicity was assessed with DAPI staining to quantify drug-specific cytotoxicity. Individual results for Samples #1 and #2 are shown as mean values from 1 experiment performed in duplicate wells, in panels A and B, respectively.