Normal B and T cells showed similar phenotypic changes when compared with CLL cells. PBMCs from 4 normal healthy donors were introduced into the circulating model in the same way as CLL PBMCs. Samples were then taken from the circulating compartment and the EVS at 48 hours and labeled with the same panel of cell surface markers used for the CLL samples. B cells were identified as CD19⁺ and T cells as CD5⁺/CD19⁻. Expression (MFI) of (A) CD62L, (B) CXCR4, (C) CD5, (D) CD49d, and (E) CD69 in circulating B and T cells is shown. (F) Normal B and T cells also migrated into the EVS, and the percentage of B-cell and T-cell migration is shown. (G) Expression of MMP-9, CD49d, CD80, CD38, CD69, and CD19 in normal B and T cells was compared in cells recovered from the EVS and cells remaining in the circulation compartment. (H) B cells from normal samples migrated significantly less than CLL B cells at 48 hours. Error bars indicate ± standard deviation. *P < .05.