Siglec-G–deficient DCs show enhanced stimulation of allogeneic T cells. BMDCs from WT-B6 and Siglec-G^−/− mice were used as stimulators in an MLR with T cells from either BALB/c (allogeneic) or C57BL/6 (syngeneic) mice and analyzed for (A) T-cell proliferation based on ³H-thymidine incorporation at 96 hours. The data are the mean ± SEM of quadruplicate cultures and are representative of 3 independent experiments. (B) Supernatants from the cultures were collected at 80 hours and analyzed for IFN-γ by ELISA. The data are the mean ± SEM of quadruplicate cultures and are from 1 of 3 similar experiments. (C) Representative figures for CFSE, and (D) Annexin-V staining of cells gated on CD90.2⁺ T cells after the MLR. CFSE-labeled splenic CD90⁺ T cells from BALB/c mice were cultured for 96 hours with BMDCs from either WT-B6 or Siglec-G^−/− mice. The cells were then harvested and analyzed. The right column shows the collective data of 3 independent experiments. N.S., no significant differences between B6 and Siglec-G^−/− DCs.