WBC and HPC mobilization effect in PB of mice injected with G-CSF, plerixafor, or Me6, alone or in combination. (A) WBCs, including (B) neutrophils and lymphocytes in the blood were measured by using a hemocytometer. (C) Total CFUs, including (D) CFU-G/GM/M, CFU-GEMM, BFU-E, and (E) HPP-CFU in PB were analyzed. Plerixafor, Me6, or G-CSF was injected into C57BL/6 mice alone or in combination. For the combination treatment, C57BL/6 mice were first injected subcutaneously with G-CSF (2.5 µg per mouse) twice a day for 4 days. Eight or 19 hours after the last injection of G-CSF, the mice were injected with 5 mg/kg of either Me6 or plerixafor; PB was harvested after 20 hours for G-CSF–treated mice, 1 hour after injection for plerixafor-treated mice, and 12 hours after injection for Me6-treated mice. n = 6 mice per group. *P < .05 and **P < .01 vs the control group; ^#P < .05 and ^##P < .01 vs the G-CSF group. A, plerixafor; G, G-CSF; M, Me6.