Dose-response effect of Me6 on the mobilization of HPCs into the blood. (A) Mobilization of WBCs, especially (B) neutrophils and lymphocytes induced by the administration of Me6 at different doses. C57BL/6 mice were injected subcutaneously with 0, 2.5, 5, or 10 mg/kg of Me6. Twelve hours after injection, the mice were euthanized and PB cells were obtained. The blood cells were counted by using a hemocytometer. n = 6 mice per group. *P < .05 and **P < .01 vs the 0-mg/kg group. (C) Dose-response analysis of total CFUs, including (D) CFU-G/GM/M, CFU-GEMM, BFU-E, and (E) HPP-CFU in PB of C57BL/6 mice 12 hours after subcutaneous injection using 0, 2.5, 5, or 10 mg/kg Me6. n = 6 mice per group. *P < .05 and **P < .01 vs the 0-mg/kg group. PB cells were obtained and evaluated by CFU assay. (F-G) The percentage analysis of LSK cells in PB and spleen of mice at 12 hours after administration of 5 mg/kg Me6 or PBS. *P < .05 vs the control group. (H) Apoptosis phenotype of PB LSK cells was assessed by using propidium iodide and Annexin-V staining. Mice were subcutaneously injected with 5 mg/kg Me6 and were euthanized 12 hours after injection. *P < .05 vs the control group. Con: control.