Fibrinolysis during GpIbα-VWF interaction blockade promotes embolization of large platelet aggregates from the thrombus and impairs distal microcirculation. (A) Representative laser Doppler profiles of mice treated with saline or ATA followed by continuous infusion of tPA. The black arrow indicates a sudden drop in CBF during tPA infusion, probably due to embolism. (B) Mean CBF at the end of the monitoring period (40 minutes posttreatment, n = 10-11). (C) Proportion of animals with occlusive thrombi on histology 1 hour after treatment injection (n = 9-12). (D) Representative multiphoton images of Evan’s blue-injected mice 1 hour after saline, ATA, or tPA-ATA treatment. Both ipsilateral and contralateral sides are represented. (E) Mean apparent vascular volume in saline-, ATA-, and tPA-ATA–treated mice 1 hour after administration (n = 4/group). ns, not significant.