CAR-T cells exposed to IL-7 and IL-15 have improved in vivo persistence and antitumor activity. (A) Schematic representation of the experiments in NSG mice to compare persistence of CAR-T cells expanded with either IL-2 or IL-7 and IL-15. (B-C) Time-course of T-cell luminescence in mice engrafted with Raji tumor cells. Data summarize 10 mice per group from 2 independent experiments. (D) Detection of CAR-T cells in the spinal cord 2 weeks after CAR-T cell adoptive transfer. Data shown are representative of 5 mice per group from 2 independent experiments. (E) Schematic representation of the experiments in NSG mice to measure the antitumor effects of CAR-T cells expanded with either IL-2 or IL-7 and IL-15. (F) Survival curve of mice after infusion of control and CAR-T cells. Data illustrate the summary of 3 independent experiments for a total of 15 mice per group.