BM pathology from NSG mice treated with 4 weeks of ESKM or isotype IgG control after human HLA-A*02:01 CD34⁺ transplant, compared with normal NSG mice. (A) No differences were seen between ESKM- and IgG-treated mice, and both groups have increased immature myeloid and erythroid lineages compared with NSG mice. The transplanted groups also have lymphocytes, which NSG mice lack. Engraftment of human cells was the same in ESKM- and IgG-treated mice, ranging from 20% to 60%. The error bars show the fifth and 95th percentiles. (B) Immunohistochemical staining of NSG mouse BM transplanted with human CD34⁺ cells for hCD45. There was no significant difference between the mice treated with ESKM (top panels) and those treated with isotype control (bottom panels). Normal NSG mice samples were did not stain positive for hCD45 (not shown).